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Flow-injection determination of glucose in serum with an immobilized pyranose oxidase reactor

✍ Scribed by Nobutoshi Kiba; Fumito Ueda; Motohisa Furusawa; Takeshi Yamane

Publisher: Elsevier Science
Year: 1992
Tongue: English
Weight: 386 KB
Volume: 269
Category: Article
ISSN: 0003-2670
DOI: 10.1016/0003-2670(92)85401-q

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✦ Synopsis

A flow-mqectlon system for the chemdummometnc determmatlon of o-glucose m serum with an lmmobdlzed pyranose oxldase reactor IS described Pyranose oxldase IS lmmobdlzed on controlled-pore glass beads and packed mto a stamless-steel column Serum (100 nl) IS dduted lOWfold with water Sample solution (10 ~1) IS Injected mto the tamer stream The hydrogen peroxrde produced IS detected by measurmg the chemdummescence emltted on adrmxmg with lummol and potassmm hexacyanoferrate(II1) The maximum sample throughput IS 60 h-' The cahbratlon graph 1s linear from 0 2 to 500 PM glucose, the detectlon hmlt IS 0 05 PM The lmmobdlzed enzyme reactor IS stable for at least 2 months

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