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Flow-injection determination of 3-hdroxybutyrate in serum with an immobilized 3-hydroxybutyrate dehydrogenase reactor and chemiluminescence detection

โœ Scribed by Nobutoshi Kiba; Hidekazu Koemado; Motoshisa Furusawa

Publisher
Elsevier Science
Year
1994
Tongue
English
Weight
414 KB
Volume
290
Category
Article
ISSN
0003-2670

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โœฆ Synopsis

A flow-injection system for the determination of 3-hydroxybutyrate in serum is described. 3-Hydroxybutyrate dehydrogenase is immobilized on poly(vinyl alcohol) beads and incorporated in a flow-injection system. 1-Methoxy-Smethylphenazinium methylsulphate reacts with enzymatically generated NADH to give H202, which is detected chemiluminometrically with the reaction of luminol and hexacyanoferrat4111). Serum is diluted and filtered through an ultrafiltration membrane. The system responds linearly to injected samples (80 ~1) in the concentration range OS-300 PM; the detection limit is 0.1 PM. The within-day relative standard deviation (n = 90) for 58 PM 3-hydroxybutyrate in serum is 0.8%. The maximum throughput is 20 samples per hour. The immobilized enzyme is stable for at least 1 month.

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