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Flow-injection determination of 1,5-anhydroglucitol in serum with an immobilized pyranose oxidase reactor and chemiluminescence detection

✍ Scribed by Nobutoshi Kiba; Fumito Ueda; Kazuya Saegusa; Yuusuke Toto; Motohisa Furusawa; Takeshi Yamane

Publisher: Elsevier Science
Year: 1993
Tongue: English
Weight: 423 KB
Volume: 271
Category: Article
ISSN: 0003-2670
DOI: 10.1016/0003-2670(93)80550-5

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✦ Synopsis

A flow-mjectlon system wth a separation column and an unmobdlzed enzyme reactor 1s described for the determmatlon of l$anhydro-o-glucitol in serum Serum samples diluted mth water are injected into an amon-exchange column (4 cm x 4 mm I d ) The eluate 1s passed through an unmobdlzed pyranose oxldase reactor (5 cm x 4 mm I d ) The hydrogen permde produced IS detected chemdurmnometncally Hnth a lummol-hexacyanoferrate(II1) reactlon The cahbratlon graph 1s hnear over the range 4 x lo-'-2 x 10m4 M, the detection hmlt 1s 2 x lo-' M The sample throughput 1s 20 h-' with a relative standard dewatlon of less than 2 5%

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