Optimisation of conditions for detection of activated oncogenes by transfection of NIH 3T3 cells
โ Scribed by Lemoine, NR; Wynford-Thomas, V; Wynford-Thomas, D
- Book ID
- 109835824
- Publisher
- Nature Publishing Group
- Year
- 1987
- Tongue
- English
- Weight
- 669 KB
- Volume
- 55
- Category
- Article
- ISSN
- 0007-0920
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๐ SIMILAR VOLUMES
The properties of NIH 3T3 cells transfected with Shope fibroma virus (SFV) DNA were investigated. Six focus-derived cell lines were established that display the following properties: a) They all contained SFV DNA sequences at early passages, b) five of them also expressed SFV RNA at early passages a
This study reports high incidence tumourigenic activity of the pSV2neo plasmid demonstrated by transfection of NIH 3T3 cells. The plasmid is frequently used as a dominant selectable marker for confirming successful gene transfection, and the findings indicate a need for caution in interpretation and