✦ LIBER ✦

Tumourigenic transformation of mouse NIH 3T3 cells by transfection with plasmid pSV2neo

✍ Scribed by Jeannie S. Wallace; Kenneth A. Fleming; Dr. David Tarin

Book ID: 104506032
Publisher: John Wiley and Sons
Year: 1988
Tongue: English
Weight: 665 KB
Volume: 156
Category: Article
ISSN: 0022-3417
DOI: 10.1002/path.1711560114

No coin nor oath required. For personal study only.

✦ Synopsis

This study reports high incidence tumourigenic activity of the pSV2neo plasmid demonstrated by transfection of NIH 3T3 cells. The plasmid is frequently used as a dominant selectable marker for confirming successful gene transfection, and the findings indicate a need for caution in interpretation and design of assays for oncogenes which use co-transfection and subsequent selection with neomycin.

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