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One-lane sequence analysis of oligodeoxyribonucleotides

✍ Scribed by Barbara J.B. Ambrose; Miguel M. Castro; Reynaldo C. Pless


Book ID
107714325
Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
836 KB
Volume
159
Category
Article
ISSN
0003-2697

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πŸ“œ SIMILAR VOLUMES


Sequence analysis of oligodeoxyribonucle
✍ J.L. Wiebers πŸ“‚ Article πŸ“… 1973 πŸ› Elsevier Science 🌐 English βš– 619 KB

## Low-resolution mass spectra have been obtained on the following general types of mono-, di-, tetra,-and hexa-deoxyribonucleotides: pX, Xp, XpY, pXpY, pXp, pXpYp, pXpYpXpY and on trifluoroacetylated XpY, YpX, pXpYpXpY, XpYpXpY, and XpYpXpYpXpY (where X and Y indicate the purine and pyrimidine nu

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A method for DNA sequencing that combines limited chemical degradation of 3'-fluorescent-labeled DNA with densitometric analysis using an automatic sequencer is presented. The DNA sequence is determined in a single electrophoretic lane by monitoring the intensities of bands representing products of