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Sequence analysis of oligodeoxyribonucleotides by mass spectrometry. 1. Dinucleoside monophosphates

✍ Scribed by Wiebers, J. L.; Shapiro, J. A.

Book ID
126444185
Publisher
American Chemical Society
Year
1977
Tongue
English
Weight
658 KB
Volume
16
Category
Article
ISSN
0006-2960

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## Low-resolution mass spectra have been obtained on the following general types of mono-, di-, tetra,-and hexa-deoxyribonucleotides: pX, Xp, XpY, pXpY, pXp, pXpYp, pXpYpXpY and on trifluoroacetylated XpY, YpX, pXpYpXpY, XpYpXpY, and XpYpXpYpXpY (where X and Y indicate the purine and pyrimidine nu

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Negative ion fast atom bombardment mass spectrornetry is used to verify the sequence of olΒ‘godeoxyri~nucleotides containing a single modified nucleotide. Both the position in the sequence and the rnolecular weight of the modified nucleotide can be determined from the 3 and 5'-phosphate sequence ions