Negative ion fast atom bombardment mass spectrornetry is used to verify the sequence of olΒ‘godeoxyri~nucleotides containing a single modified nucleotide. Both the position in the sequence and the rnolecular weight of the modified nucleotide can be determined from the 3 and 5'-phosphate sequence ions
Sequence analysis of oligodeoxyribonucleotides by mass spectrometry
β Scribed by J.L. Wiebers
- Publisher
- Elsevier Science
- Year
- 1973
- Tongue
- English
- Weight
- 619 KB
- Volume
- 51
- Category
- Article
- ISSN
- 0003-2697
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β¦ Synopsis
Low-resolution
mass spectra have been obtained on the following general types of mono-, di-, tetra,-and hexa-deoxyribonucleotides: pX, Xp, XpY, pXpY, pXp, pXpYp, pXpYpXpY and on trifluoroacetylated XpY, YpX, pXpYpXpY, XpYpXpY, and XpYpXpYpXpY (where X and Y indicate the purine and pyrimidine nucleosides, cytidine, thymidine, adenosine, or guanosine). The mass spectral fragmentation data show that:
π SIMILAR VOLUMES
Key steps in a proposed automated system for polypeptide sequencing utilizing a liquid chromatograph mass spectrometer computer system have been tested with mixtures containing up to six model oligopeptides. At the low nanomole level it was possible to obtain complete sequence information for all co
## Abstract Tandem mass spectrometry (MS/MS) is an attractive technique for sequencing membrane proteins because it can be applied to peptides in mixtures that are difficult to separate chromatographically. To evaluate the suitability of MS/MS sequencing for membrane proteins and to develop protoco