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One-Lane Chemical Sequencing of 3′-Fluorescent-Labeled DNA

✍ Scribed by S. Ferraboli; R. Negri; E. Dimauro; S. Barlati

Publisher
Elsevier Science
Year
1993
Tongue
English
Weight
449 KB
Volume
214
Category
Article
ISSN
0003-2697

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✦ Synopsis

A method for DNA sequencing that combines limited chemical degradation of 3'-fluorescent-labeled DNA with densitometric analysis using an automatic sequencer is presented. The DNA sequence is determined in a single electrophoretic lane by monitoring the intensities of bands representing products of cleavage at the four bases obtained by solvolysis in hot aqueous piperidine (10%) followed by treatment with hot formamide. An application of the method for the detection of point mutations is reported.

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