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Oncornavirus-like particles in malignant melanoma and control biopsies

✍ Scribed by P. G. Parsons; E. Klucis; P. D. Goss; J. H. Pope; J. H. Little; N. C. Davis

Publisher
John Wiley and Sons
Year
1976
Tongue
French
Weight
522 KB
Volume
18
Category
Article
ISSN
0020-7136

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✦ Synopsis

Abstract

Tests for the presence of oncornavirus‐like particles in human biopsies were made by the Spiegelman simultaneous assay for 70S RNA and RNA‐dependent DNA polymerase and by detection of 600–900S particles, incorporating ^3^H‐uridine, produced by cultured biopsy cells. Thirty‐one malignant melanoma biopsies from 29 patients were studied. Using the simultaneous assay, evidence of virus‐like particles was found in 15/26 (58%) of melanoma biopsies, 0/3 naevi pool, 1/4 samples of skin adjacent to melanoma, 0/3 samples of normal adult skin and 0/3 prepuces. The velocity sedimentation technique was shown to be a useful screening test for oncornaviruses in studies of two virus‐producing mouse cell lines (TKL‐5 and WEHI‐22), and was positive with 7/9 melanoma biopsies. Overall, these results are compatible with the earlier findings of similar virus‐like particles in malignant melanoma cell lines, but the exact nature of the particles remains to be defined.

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