𝔖 Bobbio Scriptorium
✦   LIBER   ✦

Oncornavirus-like particles in HeLa cells. II. Immunological characterization of the virus

✍ Scribed by Heinz Bauer; Johan Hendrik Daams; Kenneth F. Watson; Karin Mölling; Hans Gelderblom; Werner Schäfer

Publisher
John Wiley and Sons
Year
1974
Tongue
French
Weight
579 KB
Volume
13
Category
Article
ISSN
0020-7136

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

An oncornavirus‐like agent has been described in HeLa cells of human origin (Gelderblom et al., 1974). The investigation of its protein constituents revealed a pattern similar to but different from that of known oncornaviruses. Six polypeptides with molecular weights (mw) between 12,000 d and 28,000 d, and two glycopolypeptides of about 60,000 d and 80,000 d mw were detected by SDS‐polyacrylamide gel electrophoresis. No serological cross‐reaction was found between the HeLa virus and B‐ and C‐type oncornaviruses. Essentially, none of two interspecies‐specific antigenic determinants described in mammalian C‐type viruses has been detected in the HeLa virus. However a distinct serological relationship was found between the HeLa virus and the Mason‐Pfizer monkey virus in that these viruses share at least two antigens in the mw range of 16,000 d and 28,000 d, respectively. These results confirm the electron microscopic data described before (Gelderblom et al., 1974) and extend the evidence for a new group of oncornavirus‐like particles.

📜 SIMILAR VOLUMES

Oncornavirus-like particles in HeLa cell
Oncornavirus-like particles in HeLa cells. III. Biochemical characterization of the virus
✍ Kenneth F. Watson; Karin Mölling; Hans Gelderblom; Heinz Bauer 📂 Article 📅 1974 🏛 John Wiley and Sons 🌐 French ⚖ 419 KB

## Abstract Oncornavirus‐like particles observed and purified from HeLa cell culture supernates have a density in sucrose of 1.16‐1.17 g/cm^3^. They contain DNA polymerase and ribonuclease activities with properties characteristic of reverse transcriptase and ribonuclease H of known oncornaviruses.

Detection of oncornavirus-like particles
Detection of oncornavirus-like particles in HeLa cells. I. Fine structure and comparative morphological classification
✍ Hans Gelderblom; Heinz Bauer; Hajime Ogura; Reinhard Wigand; Anna B. Fischer 📂 Article 📅 1974 🏛 John Wiley and Sons 🌐 French ⚖ 743 KB

## Abstract Oncornavirus‐like particles have been consistently observed over a period of 3 years in HeLa cells obtained from several laboratories. The particles mature by budding, in that previously fully assembled A‐type‐like particles are enveloped by the protruding cell membrane with subsequent

Optimization of the production of virus-
Optimization of the production of virus-like particles in insect cells
✍ Pedro E. Cruz; António Cunha; Cristina C. Peixoto; João Clemente; José L. Moreir 📂 Article 📅 1998 🏛 John Wiley and Sons 🌐 English ⚖ 345 KB 👁 1 views

In this work the maximal operational hydrodynamic conditions (agitation and aeration rate) that cause no adverse effect in Sf-9 cells growth in SF900II serumfree medium were determined. Shear stresses higher than 1 N m -2 and aeration rates higher than 0.04 vvm affect cell growth and when these cond

Immunological mechanisms in the pathogen
Immunological mechanisms in the pathogenesis of virus-induced murine leukemia. II. Characterization of autoreactive thymocytes
✍ Max R. Proffitt; Martin S. Hirsch; Ian F. C. McKenzie; Beatriz Gheridian; Paul H 📂 Article 📅 1975 🏛 John Wiley and Sons 🌐 French ⚖ 786 KB

## Abstract The nature of the reaction and the type of effector cells involved in the reactivity of thymocytes from Moloney murine leukemia virus (MuLV‐M)‐carrier mice against normal syngeneic target cells has been further characterized. The reaction is mediated by viable MuLV‐infected thymocytes.

Characterization of the receptor for ins
Characterization of the receptor for insulin-like growth factor II in bone cells
✍ Subburaman Mohan; Thomas Linkhart; Ron Rosenfeld; David Baylink 📂 Article 📅 1989 🏛 John Wiley and Sons 🌐 English ⚖ 937 KB

We have previously shown that insulin-like growth factor II (IGF-II) is produced by bone cells and that IGF-II stimulates cell proliferation and collagen synthesis in bone cells. We now extend these in vitro findings by demonstrating specific IGF-I1 binding to bone cells derived from newborn mouse c