On the use of carboxamidomethyl esters in the protease-catalyzed peptide synthesis
β Scribed by Peter Kuhl; Ute Zacharias; Helmut Burckhardt; Hans-Dieter Jakubke
- Publisher
- Springer Vienna
- Year
- 1986
- Tongue
- English
- Weight
- 422 KB
- Volume
- 117
- Category
- Article
- ISSN
- 0026-9247
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π SIMILAR VOLUMES
Papain catalyzed synthesis of glyceryl esters of BOC(Z)-protected amino acids and peptides was performed at 40-50Β°C in a 50 molar excess of glycerol. Equilibrium was achieved in 6-7 h. The maximal yield of esters (50-70%) was obtained at 10% of water and pH 3.2-3.4. A lower water concentration resul
Benzyloxycarbonyl-L-proline p-guanidinophenyl ester is an "inverse substrate" for trypsin; i.e., the cationic center is included in the leaving group instead of being in the acyl moiety. This substrate can be used in trypsin-catalyzed acyl-transfer reactions leading to the sythesis of Pro-Xaa peptid