Protease-catalyzed peptide synthesis using inverse substrates: The influence of reaction conditions on the trypsin acyl transfer efficiency

Protease-catalyzed peptide synthesis usi

Protease-catalyzed peptide synthesis using inverse substrates: The synthesis of Pro-Xaa-bonds by trypsin

✍ Volker Schellenberger; Ute Schellenberger; Hans-Dieter Jakubke; Nina P. Zapevalo 📂 Article 📅 1991 🏛 John Wiley and Sons 🌐 English ⚖ 296 KB

Benzyloxycarbonyl-L-proline p-guanidinophenyl ester is an "inverse substrate" for trypsin; i.e., the cationic center is included in the leaving group instead of being in the acyl moiety. This substrate can be used in trypsin-catalyzed acyl-transfer reactions leading to the sythesis of Pro-Xaa peptid