The kinetics of the losses of thiamin, niacinamide, and riboflavin were investigated during the cooking of macaroni at 50,75, 80, 85, and 90 "C. Simultaneous analysis of the vitamins was achieved by HPLC using a p-Bondapak column. The activation energies for the losses of thiamin, niacinamide, and r
On the loss of vitamin B2 from yeast during dissimilation
โ Scribed by Stier, T. J. B. ;Macintyre, Claire F.
- Publisher
- Wiley (John Wiley & Sons)
- Year
- 1942
- Tongue
- English
- Weight
- 469 KB
- Volume
- 20
- Category
- Article
- ISSN
- 0095-9898
No coin nor oath required. For personal study only.
โฆ Synopsis
Our inaiii object in beginning the work reported herein was t o find out how to modify the concentration of riboflavin in yeast cells. Some experiments of Stier ('39) indicated that this should be the next step in the investigation of carbohydrate and lipid synthesis of yeast, since it seemed likely from his work that riboflavin played an important role in aerobic assimilation.
Factors which would induce non-proliferating yeast cells to lose riboflavin were sought. Since Pett ('35) had reported a loss of riboflavin during aeration in phosphate buffer solutions we began by investigating the time-course of riboflavin loss under similar experimental conditions. The development of vitamin B, deficiency was studied at a cellular level and particular attention was paid to the individual cellular variation in "resistance" to its onset.
' W e nisli to thank Dr. D. F. Robertson of Merrk and Company for the ribofla~in nhicli he so kindly provided.
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