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On the functional role of the Tyr-639 residue of bacteriophage T7 RNA polymerase

โœ Scribed by V.O. Rechinsky; D.A. Kostyuk; V.L. Tunitskaya; S.N. Kochetkov


Book ID
115926660
Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
355 KB
Volume
306
Category
Article
ISSN
0014-5793

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Random mutagenesis of the gene for bacteriophage T7 RNA polymerase was used to identify functionally essential amino acid residues of the enzyme. A two-plasmid system was developed that permits the straightforward isolation of T7 RNA polymerase mutants that had lost almost all catalytic activity. It