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4952496 Cloning and expression of the gene for bacteriophage T7 RNA polymerase

✍ Scribed by Grilj, V.; Skukan, N.; Jakšić, M.; Kada, W.; Kamiya, T.


Book ID
122518764
Publisher
Elsevier Science
Year
1991
Tongue
English
Weight
66 KB
Volume
9
Category
Article
ISSN
0734-9750

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Random mntagenesis of the gene for bacte
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Random mutagenesis of the gene for bacteriophage T7 RNA polymerase was used to identify functionally essential amino acid residues of the enzyme. A two-plasmid system was developed that permits the straightforward isolation of T7 RNA polymerase mutants that had lost almost all catalytic activity. It