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Construction and expression of a modular gene encoding bacteriophage T7 RNA polymerase

✍ Scribed by Nadège Arnaud; Valérie Cheynet; Guy Oriol; Bernard Mandrand; François Mallet

Book ID
117522619
Publisher
Elsevier Science
Year
1997
Tongue
English
Weight
293 KB
Volume
199
Category
Article
ISSN
0378-1119

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Random mntagenesis of the gene for bacte
Random mntagenesis of the gene for bacteriophage T7 RNA polymerase
✍ Rechinsky, Vladimir O. ;Kostyuk, Dmitry A. ;Lyakhov, Dmitry L. ;Chernov, Boris K 📂 Article 📅 1993 🏛 Springer 🌐 English ⚖ 421 KB

Random mutagenesis of the gene for bacteriophage T7 RNA polymerase was used to identify functionally essential amino acid residues of the enzyme. A two-plasmid system was developed that permits the straightforward isolation of T7 RNA polymerase mutants that had lost almost all catalytic activity. It