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Nucleotide sequence of the cloned gene for bacteriophage T7 RNA polymerase

โœ Scribed by Stephen J. Stahl; Kai Zinn


Book ID
118918275
Publisher
Elsevier Science
Year
1981
Tongue
English
Weight
367 KB
Volume
148
Category
Article
ISSN
0022-2836

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Random mutagenesis of the gene for bacteriophage T7 RNA polymerase was used to identify functionally essential amino acid residues of the enzyme. A two-plasmid system was developed that permits the straightforward isolation of T7 RNA polymerase mutants that had lost almost all catalytic activity. It