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Mutations induced by bacteriophage T7 RNA polymerase and their effects on the composition of the T7 genome

✍ Scribed by Anton Beletskii; Andrei Grigoriev; Susan Joyce; Ashok S Bhagwat

Book ID
115630401
Publisher
Elsevier Science
Year
2000
Tongue
English
Weight
250 KB
Volume
300
Category
Article
ISSN
0022-2836

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## Abstract Bacteriophage T3‐induced RNA polymerase is rapidly inactivated at 42Β°C. Addition of T3 DNA delays this process for 30 s and reduces the rate with which the enzyme activity is lsot indicating that a labile binary complex between T3 DNA and polymerase must have been formed. The ternary co