On the colorimetric biuret method of protein determination

✍ Scribed by R. Parvin; S.V. Pande; T.A. Venkitasubramanian

• Book ID: 102982669
• Publisher: Elsevier Science
• Year: 1965
• Tongue: English
• Weight: 651 KB
• Volume: 12
• Category: Article
• ISSN: 0003-2697
• DOI: 10.1016/0003-2697(65)90085-0

✦ Synopsis

The calorimetric biuret procedure has been widely employed for estimation of protein from a variety of biological materials but studies concerning the limitations and validity of this method have been few. Pande et al. (1) noted that, for extracts of Aspergillus niger, the biuret method of Gornall et al. (2) was not as satisfactory as the phenol method of Lowry et al. (3). Khanna et al. ( 4) reported that the use of the biuret procedure gave misleading high values for proteins in different biological extracts. The present study confirms and amplifies the above observations. In addition, experiments pertaining to the elucidat,ion of the cause of interference in the biuret method, its removal, and the applicability of the phenol method are also described. While this manuscript was in preparation a study of similar nature appeared (5).

Methods

Chemicals. Sufficiently pure chemicals of either British Drug House, England, or E. Merck, Germany, were used. Bovine serum albumin (BSA) (fraction V) was a product of Sigma Chemical Co.