Determination of proteins in “Tris” buffer by the biuret reaction
✍ Scribed by R.M. Robson; D.E. Goll; M.J. Temple
- Publisher
- Elsevier Science
- Year
- 1968
- Tongue
- English
- Weight
- 166 KB
- Volume
- 24
- Category
- Article
- ISSN
- 0003-2697
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A method for quantitative determination of insoluble proteins by means of the biuret reaction is proposed. The sample is oxidized with excess HIOZ, dried, and subsequently dissolved in 0.05% sodium dodecyl sulfate-1 N NaOH at 1WC. The clear solution obtained is treated with biuret reagent and the ab
## Abstract A biuret automated colorimetric assay for total protein in urine and cerebrospinal fluids was established. The procedures were as follows. Acidify all urine sample before analysis. Add precipitant Na~2~WO~4~ to urine samples. After 10 min, centrifuge, decant the supernatant fluid, drain