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Extending the range of application of the biuret reaction: Quantitative determination of insoluble proteins

✍ Scribed by I. Goshev; P. Nedkov

Publisher: Elsevier Science
Year: 1979
Tongue: English
Weight: 261 KB
Volume: 95
Category: Article
ISSN: 0003-2697
DOI: 10.1016/0003-2697(79)90736-x

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✦ Synopsis

A method for quantitative determination of insoluble proteins by means of the biuret reaction is proposed. The sample is oxidized with excess HIOZ, dried, and subsequently dissolved in 0.05% sodium dodecyl sulfate-1 N NaOH at 1WC. The clear solution obtained is treated with biuret reagent and the absorbance of the chromogen is measured at 3540°C. The method is applied successfully to collagen from bovine Achilles tendon, keratin of sheep's wool, and defatted sheepskin. Biuret chromogen absorbance of HZOg-oxidized human serum albumin is 10 f 0~5% lower than that obtained with the native protein.

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