The calorimetric biuret procedure has been widely employed for estimation of protein from a variety of biological materials but studies concerning the limitations and validity of this method have been few. Pande et al. (1) noted that, for extracts of Aspergillus niger, the biuret method of Gornall e
β¦ LIBER β¦
Effect of glycerol on Lowry and biuret methods of protein determination
β Scribed by M.K. Zishka; J.S. Nishimura
- Book ID
- 107711074
- Publisher
- Elsevier Science
- Year
- 1970
- Tongue
- English
- Weight
- 300 KB
- Volume
- 34
- Category
- Article
- ISSN
- 0003-2697
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Certain reagents utilized in the formation of polyacrylamide gels are shown to interfere in the Lowry assay for protein. Acrylamide (3-30%) and potassium ferrocyanide (O.OOlS-0.0105%) produced a linear response in color formation. Both compounds are capable of reducing the phenol reagent in the abse