On the binding of ethidium bromide in synthetic polyelectrolyte solutions

✍ Scribed by V. Crescenzi; F. Quadrifoglio

• Publisher: Elsevier Science
• Year: 1974
• Tongue: English
• Weight: 399 KB
• Volume: 10
• Category: Article
• ISSN: 0014-3057
• DOI: 10.1016/0014-3057(74)90126-8

✦ Synopsis

Results are reported for spectroscopic and calorimetric studies of ethidium bromide (EBr) in water and in aqueous solutions of three alternating maleic acid copolymers. The dimerization constant, dimerizafion enthalpy and the dimer spectrum of EBr are derived. The interaction of EBr with the polycarboxylates is characterized on the basis of spectral and microcalorimetric data according to two limiting mechanisms: at low dye-to-polymer concentration ratios, EBr would be bound essentially in monomeric form with small exothermic effects: at high concentration ratios, the observed comparatively large exothermic heat is attributed to bound dye stacking.