Action of ethidium bromide on mitochondrial DNA in the petite-negative yeast Schizosaccharomyces pombe
โ Scribed by Bandlow, Wolfhard ;Kaudewitz, Fritz
- Publisher
- Springer
- Year
- 1974
- Tongue
- English
- Weight
- 387 KB
- Volume
- 131
- Category
- Article
- ISSN
- 0026-8925
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A population of small covalently closed non-mitochondrial circular DNA molecules was isolated from the petite-negative yeast Schizosaccharomyces pombe. The mean length of these molecules, possessing the same density as nuclear DNA (1.695 g/cm3) is 1.95 +/- 0.18 micrometer. The presence of these mini
Size and shape of mitochondrial DNA molecules of Schizosaccharomyces pombe were analyzed by electron microscopy. Besides numerous linear molecules, circular molecules ranging from 0.83 micron to 12.81 micron were found. Depending on the method of preparation, both closed and open circular molecules
Treatment of haploid strains of the petite negative yeast Schizosaceharornyces pombe with ethidium bromide prior to mating with untreated cells reduces transmission ofmitochondrial markers from the treated strains. This effect is fully reversible after 20 generations of growth in drug free medium be