2 μm Covalently closed non-mitochondrial circular DNA in the petite-negative yeast Schizosaccharomyces pombe

✍ Scribed by Del Giudice, L. ;Wolf, K. ;Sassone-Corsi, P. ;Mazza, A.

• Publisher: Springer
• Year: 1979
• Tongue: English
• Weight: 497 KB
• Volume: 172
• Category: Article
• ISSN: 0026-8925
• DOI: 10.1007/bf00268278

✦ Synopsis

A population of small covalently closed non-mitochondrial circular DNA molecules was isolated from the petite-negative yeast Schizosaccharomyces pombe. The mean length of these molecules, possessing the same density as nuclear DNA (1.695 g/cm3) is 1.95 +/- 0.18 micrometer. The presence of these minicircles in crude mitochondrial preparations indicates their tight association with mitochondrial particles. Their disappearance after DNase treatment of mitochondria demonstrates their extramitochondrial location.