𝔖 Bobbio Scriptorium
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Effect of ethidium bromide on transmission of mitochondrial genomes and DNA synthesis in the petite negative yeastSchizosaccharomyces pomhe

✍ Scribed by K. Wolf; L. Giudice

Publisher
Springer-Verlag
Year
1980
Tongue
English
Weight
387 KB
Volume
1
Category
Article
ISSN
0172-8083

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✦ Synopsis

Treatment of haploid strains of the petite negative yeast Schizosaceharornyces pombe with ethidium bromide prior to mating with untreated cells reduces transmission ofmitochondrial markers from the treated strains. This effect is fully reversible after 20 generations of growth in drug free medium before mating. In contrast to the petite positive yeast Saccharornyces cerevisiae, where nuclear DNA synthesis is not affected but mitochondrial DNA is degraded in the presence of 20 /~g/ml ethidinm bromide, the same concentration decreases both nuclear and mitochondrial DNA synthesis in Schizosaccharomyces pombe. After removal of the drug, nuclear DNA synthesis increases faster than its mitochondrial counterpart in Schizosaccharomyces pombe.

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A series of mutants called ebi, less inducible by ethidium bromide than the parental strain for the rho+ leads to rho- mutation have been isolated after E.M.S. mutagenesis. Some of the ebi mutants also show an important accumulation of rho- cells, in the absence of ethidium bromide. Ebi mutations ar