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Effect of caffeine on the rho--induction with ethidium bromide in Saccharomyces cerevisiae

✍ Scribed by Wolf, K. ;Kaudewitz, F.

Publisher
Springer
Year
1976
Tongue
English
Weight
403 KB
Volume
146
Category
Article
ISSN
0026-8925

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✦ Synopsis

It is shown that caffeine antagonizes petite-induction with ethidium bromide under non-growth conditions when administered during or after mutagenic treatment. Caffeine itself is shown to be a petite-inducing agent when cells are grown in liquid glucose-complete-medium in the presence of the drug. A possible mode of action of caffeine in the ethidium bromide induced petite-mutagenesis is discussed.

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The addition of 0.1% caffeine to the plating medium markedly reduced the ozone-survival of the wild-type and the rad1 and rad6 mutants of Saccharomyces cerevisiae, whereas no effect was observed in the rad52 mutant. Since, in S. cerevisiae, caffeine has been reported to interfere with the recombinat