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On-line immunoanalysis of monoclonal antibodies during a continuous culture of hybridoma cells

✍ Scribed by Jens J. van der Pol; Marc Machnik; Manfred Biselli; Theresa Portela-Klein; Cornelis D. de Gooijer; Johannes Tramper; Christian Wandrey

Book ID
110256159
Publisher
Springer
Year
1997
Tongue
English
Weight
119 KB
Volume
24
Category
Article
ISSN
0920-9069

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## Abstract The flow‐cytometric (FCM) analysis of bivariate DNA/lgG distributions has been conducted to study the cell cycle kinetics and monoclonal antibody (MAb) production during perfusion culture of hybridoma cells. Three different perfusion rates were employed to demonstrate the dependency of