Perfusion Cultures of Hybridoma Cells for Monoclonal Antibody Production
β Scribed by FRANCISCO J. CASTILLO; LARRY J. MULLEN; JOHN C. THRIFT; BARRY C. GRANT
- Book ID
- 119867862
- Publisher
- John Wiley and Sons
- Year
- 1992
- Tongue
- English
- Weight
- 393 KB
- Volume
- 665
- Category
- Article
- ISSN
- 0890-6564
No coin nor oath required. For personal study only.
π SIMILAR VOLUMES
A fixed bed perfusion system for hybridoma cell immobilization is presented. The system consists of a culturing vessel (300 ml total volume) in which polyurethane (PU) sponges in the form of small cubes of about 5 mm sides are packed. Cells are immobilized by physical entrapment in the foam matrix.
## Abstract The flowβcytometric (FCM) analysis of bivariate DNA/lgG distributions has been conducted to study the cell cycle kinetics and monoclonal antibody (MAb) production during perfusion culture of hybridoma cells. Three different perfusion rates were employed to demonstrate the dependency of