To determine the effect of hyperosmotic stress on the monoclonal antibody (MAb) production by calciumalginate-immobilized S3H5iy2bA2 hybridoma cells, the osmolalities of medium in the M A b production stage were varied through the addition of NaCI. The specific MAb productivity (9Mab) of immobilized
Dialysis cultures with immobilized hybridoma cells for effective production of monoclonal antibodies
✍ Scribed by Ralf Pörtner; Ines Lüdemann; Herbert Märkl
- Book ID
- 110256135
- Publisher
- Springer
- Year
- 1997
- Tongue
- English
- Weight
- 119 KB
- Volume
- 23
- Category
- Article
- ISSN
- 0920-9069
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A fixed bed perfusion system for hybridoma cell immobilization is presented. The system consists of a culturing vessel (300 ml total volume) in which polyurethane (PU) sponges in the form of small cubes of about 5 mm sides are packed. Cells are immobilized by physical entrapment in the foam matrix.
## Abstract Cell proliferation and long‐term production of monoclonal antibody IgG~2b~ by M2139 hybridoma cells immobilized in macroporous gel particles (MGPs) in packed‐bed reactor were studied for a period of 60 days. The MGPs were made of supermacroporous gels produced in frozen conditions from