Hybridoma growth and monoclonal antibody production in a dialysis perfusion system
β Scribed by B. Amos; M. Al-Rubeai; A.N. Emery
- Publisher
- Elsevier Science
- Year
- 1994
- Tongue
- English
- Weight
- 816 KB
- Volume
- 16
- Category
- Article
- ISSN
- 0141-0229
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## Abstract The flowβcytometric (FCM) analysis of bivariate DNA/lgG distributions has been conducted to study the cell cycle kinetics and monoclonal antibody (MAb) production during perfusion culture of hybridoma cells. Three different perfusion rates were employed to demonstrate the dependency of
A quantitative study of the influence of initial serum concentration on hybridoma growth rate, maximum viable and total cell yield, and specific antibody production rate is presented. The specific growth rate varied in a Monod fashion with initial serum levels (2-10Β°/0 FCS), giving K,,, = 1.6 v/v% a