A flow-cytometric analysis of hybridoma growth and monoclonal antibody production
โ Scribed by Mina Dalili; David F. Ollis
- Publisher
- John Wiley and Sons
- Year
- 1990
- Tongue
- English
- Weight
- 678 KB
- Volume
- 36
- Category
- Article
- ISSN
- 0006-3592
No coin nor oath required. For personal study only.
๐ SIMILAR VOLUMES
An attempt has been made to mathematically describe and analyze monoclonal antibody (MAB) productivity of hybridoma cells, with particular emphasis on continuous cultures under unsteady-state conditions. A simple and unstructured general kinetic model that takes account of productivity loss during l
A quantitative study of the influence of initial serum concentration on hybridoma growth rate, maximum viable and total cell yield, and specific antibody production rate is presented. The specific growth rate varied in a Monod fashion with initial serum levels (2-10ยฐ/0 FCS), giving K,,, = 1.6 v/v% a
## Abstract **BACKGROUND:** Many reports have suggested that mild hypothermic culture conditions improve the specific monoclonal antibody (mAb) productivity of mammalian cells. The effect of active hypothermic growth on the mAb productivity of the hybridoma C~2~E~7~ was investigated. Hybridoma grow