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Effect of dissolved oxygen concentration on monoclonal antibody production in hybridoma cell cultures

✍ Scribed by Tatsuya Ogawa; Masamichi Kamihira; Hitoshi Yoshida; Shinji Iijima; Takeshi Kobayashi

Book ID
113396725
Publisher
Elsevier Science
Year
1992
Tongue
English
Weight
558 KB
Volume
74
Category
Article
ISSN
0922-338X

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Effects of dissolved oxygen concentratio
Effects of dissolved oxygen concentration on hybridoma growth and metabolism in continuous culture
✍ William M. Miller; Charles R. Wilke; Harvey W. Blanch πŸ“‚ Article πŸ“… 1987 πŸ› John Wiley and Sons 🌐 English βš– 725 KB

Oxygen transport is a major limitation in large-scale mammalian cell culture. The effects of the dissolved oxygen concentration (DO; from 0.1 to 100% saturation with air) on Sp2/0-derived mouse hybridomas were investigated using continuous culture. The steady-state concentration of viable cells incr