Oligonucleotide labelling: A concise synthesis of a modified thymidine phosphoramidite
โ Scribed by Cruickshank Kenneth A; Stockwell Daniel L
- Book ID
- 108381761
- Publisher
- Elsevier Science
- Year
- 1988
- Tongue
- French
- Weight
- 267 KB
- Volume
- 29
- Category
- Article
- ISSN
- 0040-4039
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๐ SIMILAR VOLUMES
The synthesis of a benzotriazole azo dye phosphoramidite and the subsequent use in solid phase synthesis of oligonucleotides is reported. The azo dye is shown as a surface enhanced resonance Raman label for oligonucleotides that is capable of immobilisation of the oligonucleotide on metal surfaces s
A method for the addition of a benzotriazole moiety to the 5%-terminus of an oligonucleotide via phosphoramidite chemistry has been developed. Use of a monomethoxytrityl protecting group on the benzotriazole allowed fast on-column detritylation purification by reverse-phase HPLC. Surface enhanced Ra