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Synthesis of a benzotriazole phosphoramidite for attachment of oligonucleotides to metal surfaces

โœ Scribed by Rachel Brown; W.Ewen Smith; Duncan Graham

Publisher
Elsevier Science
Year
2001
Tongue
French
Weight
86 KB
Volume
42
Category
Article
ISSN
0040-4039

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โœฆ Synopsis

A method for the addition of a benzotriazole moiety to the 5%-terminus of an oligonucleotide via phosphoramidite chemistry has been developed. Use of a monomethoxytrityl protecting group on the benzotriazole allowed fast on-column detritylation purification by reverse-phase HPLC. Surface enhanced Raman scattering (SERS) of the modified oligonucleotides was obtained from silver colloid.

๐Ÿ“œ SIMILAR VOLUMES

Synthesis of a benzotriazole azo dye pho
Synthesis of a benzotriazole azo dye phosphoramidite for labelling of oligonucleotides
โœ Rachel Brown; W.Ewen Smith; Duncan Graham ๐Ÿ“‚ Article ๐Ÿ“… 2003 ๐Ÿ› Elsevier Science ๐ŸŒ French โš– 177 KB

The synthesis of a benzotriazole azo dye phosphoramidite and the subsequent use in solid phase synthesis of oligonucleotides is reported. The azo dye is shown as a surface enhanced resonance Raman label for oligonucleotides that is capable of immobilisation of the oligonucleotide on metal surfaces s