Oligolamellar vesicles for covalent immobilization and stabilization of d-amino acid oxidase
β Scribed by Yoshimoto, Makoto; Yamasaki, Miku; Okamoto, Masakazu; Umakoshi, Hiroshi; Kuboi, Ryoichi
- Book ID
- 119238108
- Publisher
- Elsevier Science
- Year
- 2013
- Tongue
- English
- Weight
- 556 KB
- Volume
- 52
- Category
- Article
- ISSN
- 0141-0229
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π SIMILAR VOLUMES
## Background Immobilization of __Trigonopsis variabilis__ D-amino acid oxidase (__Tv__DAO) on solid support is the key to a reasonably stable performance of this enzyme in the industrial process for the conversion of cephalosporin C as well as in other biocatalytic applications. ## Results To pr
The preparation of flavin adenine dinucleotide-aftinity columns employing glucose oxidase and D-amino acid oxidase covalently linked to Sepharose-4B is described. Both immobilized enzymes have very good long-term stabilities, retaining at least half of their original flavin adenine dinucleotide-bind