✦ LIBER ✦
Immobilized glucose oxidase and d-amino acid oxidase: A convenient method for the purification of flavin adenine dinucleotide and its analogs
✍ Scribed by Vincent Massey; Loren D. Mendelsohn
- Publisher
- Elsevier Science
- Year
- 1979
- Tongue
- English
- Weight
- 330 KB
- Volume
- 95
- Category
- Article
- ISSN
- 0003-2697
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✦ Synopsis
The preparation of flavin adenine dinucleotide-aftinity columns employing glucose oxidase and D-amino acid oxidase covalently linked to Sepharose-4B is described. Both immobilized enzymes have very good long-term stabilities, retaining at least half of their original flavin adenine dinucleotide-binding capacity over 11 months. In both cases pure flavin adenine dinucleotide is obtained readily in good yields.