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Observations on the contraction and movement of fat-storing cells in culture

✍ Scribed by Wang Xian-en; Li Ming-zhen; Ye Wang-yun

Book ID
112927126
Publisher
Springer-Verlag
Year
1994
Tongue
Chinese
Weight
668 KB
Volume
14
Category
Article
ISSN
0510-9752

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Fat-storing cells (perisinusoidal stellate cells) were isolated by enzymatic digestion of rat liver and purified by a single-step Nycodenz@ gradient to yield 11.4.10' cells per liver, with a purity of 74% and a viability of 76%. Monolayer cultures of fat-storing cells incorporated both [3SS]sulfate