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Kupffer cell-mediated induction of synthesis and secretion of proteoglycans by rat liver fat-storing cells in culture

✍ Scribed by Gressner, Axel M.; Zerbe, Ottwin


Book ID
121907734
Publisher
Elsevier Science
Year
1987
Tongue
English
Weight
867 KB
Volume
5
Category
Article
ISSN
0168-8278

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The synthesis of proteoglycans in fat-st
✍ Stephan SchΓ€fer; Ottwin Zerbe; Professor Axel M. Gressner πŸ“‚ Article πŸ“… 1987 πŸ› John Wiley and Sons 🌐 English βš– 987 KB

Fat-storing cells (perisinusoidal stellate cells) were isolated by enzymatic digestion of rat liver and purified by a single-step Nycodenz@ gradient to yield 11.4.10' cells per liver, with a purity of 74% and a viability of 76%. Monolayer cultures of fat-storing cells incorporated both [3SS]sulfate