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Comparison of proteoglycan synthesis and secretion in rat liver fat storing cells, kupffer cells and hepatocytes in culture


Book ID
123304058
Publisher
Elsevier Science
Year
1988
Tongue
English
Weight
85 KB
Volume
7
Category
Article
ISSN
0168-8278

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The synthesis of proteoglycans in fat-st
✍ Stephan SchΓ€fer; Ottwin Zerbe; Professor Axel M. Gressner πŸ“‚ Article πŸ“… 1987 πŸ› John Wiley and Sons 🌐 English βš– 987 KB

Fat-storing cells (perisinusoidal stellate cells) were isolated by enzymatic digestion of rat liver and purified by a single-step Nycodenz@ gradient to yield 11.4.10' cells per liver, with a purity of 74% and a viability of 76%. Monolayer cultures of fat-storing cells incorporated both [3SS]sulfate