Nucleotide Coupling in Reverse Micelles

✍ Scribed by Christof Böhler; Pier Luigi Luisi; Willi Bannwarth; Mauro Giustini

• Publisher: John Wiley and Sons
• Year: 1993
• Tongue: German
• Weight: 629 KB
• Volume: 76
• Category: Article
• ISSN: 0018-019X
• DOI: 10.1002/hlca.19930760321

✦ Synopsis

Nucleotide coupling was investigated in reverse micelles formed by (cety1)trimethylammonium bromide (CTAB), in hexane/pentan-1-01. In particular, the coupling of 2'-deoxy-5'-0-methylcytidine 3'-0-phosphate, prepared by phosphoramidite chemistry, with 5'-amino-5'-deoxythymidine was studied in the presence of a H20-soluble carbodiimide at w, = 11 and 22 (w, = [H,O]/[CTAB]). The effect of w, on the reaction rate was investigated. A solid-phase strategy was developed for the synthesis of 2'-deoxy-5'-0-methyl-cytidyl-(3'-5')-5'amino-5'-deoxythymidine. The nucleotide coupling yielding the expected product occurred readily in reverse micelles. Nucleotide coupling is thus possible in reverse micelles, and this is discussed in connection with the micellar self-replication program.