Nucleotide analogues as probes for DNA polymerases
โ Scribed by K.-H. Jung; A. Marx
- Publisher
- Springer
- Year
- 2005
- Tongue
- English
- Weight
- 608 KB
- Volume
- 62
- Category
- Article
- ISSN
- 1420-682X
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## Abstract For Abstract see ChemInform Abstract in Full Text.
DNA polymerase enzymes process their natural substrates with very high specificity. Yet recent experiments have shown that these enzymes can also process DNA in which the backbone or bases are modified to a surprising degree. Such experiments have important implications in understanding the mechanis
Synthesis of radiolabeled DNA probes via polymerase chain reaction (PCR) is a convenient alternative to the more conventional methods of random primer-labeling and nick translation. PCR requires less template and allows the synthesis of radiolabeled probes from specific sequences contained within cl