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Probing DNA Polymerase Function with Synthetic Nucleotides

โœ Scribed by Andreas Marx; Ilka Detmer; Jens Gaster; Daniel Summerer


Publisher
John Wiley and Sons
Year
2004
Weight
69 KB
Volume
35
Category
Article
ISSN
0931-7597

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Synthesis of radiolabeled DNA probes via polymerase chain reaction (PCR) is a convenient alternative to the more conventional methods of random primer-labeling and nick translation. PCR requires less template and allows the synthesis of radiolabeled probes from specific sequences contained within cl