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Nucleolar RNA synthetic activity in chinese hamster cells in vitro and the effects of actinomycin D and nogalamycin

✍ Scribed by Frances E. Arrighi

Publisher: John Wiley and Sons
Year: 1967
Tongue: English
Weight: 582 KB
Volume: 69
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1040690107

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✦ Synopsis

RNA synthesis was studied by autoradiographic analysis using tritiated uridine incorporation in the Chinese hamster cell line Dede after a one-minute pulse labeling period. RNA synthesis continues during all stages of interphase and mitosis except during metaphase and anaphase. Cytoplasmic RNA was apparently synthesized in the nucleus, since no grains were observed above the background level in the sample immediately following the labeling. Nucleoli synthesize their own RNA and are not reservoirs for RNA synthesized elsewhere. Both actinomycin D and nogalamycin inhibited the RNA synthetic activity of chromatin and nucleoli. However, the nucleolar synthetic activity was more susceptible to these agents than that of chromatin. Furthermore, actinomycin D was a stronger inhibitor than nogalamycin.

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