✦ LIBER ✦

A comparative study of the effects of lucanthone (miracil D) and actinomycin d on the chinese hamster cells grown in cultures

✍ Scribed by Olga I. Epifanova; G. F. Makarova; M. K. Abuladze

Publisher: John Wiley and Sons
Year: 1975
Tongue: English
Weight: 578 KB
Volume: 86
Category: Article
ISSN: 0021-9541
DOI: 10.1002/jcp.1040860210

No coin nor oath required. For personal study only.

✦ Synopsis

Abstract

Chinese hamster cells of an established clone line grown in monolayers were incubated for up to two hours with either lucanthone (0.3–30 m̈g/ ml) or actinomycin D (0.06–0.10 m̈g/ml) and subjected to radioautographic investigations with ^3^H‐uridine during the period of treatment.

At concentration of 9 m̈g/ml lucanthone selectively inhibited the synthesis of nucleolar (ribosomal) RNA while the extranucleolar RNA synthesis proceeded at a high level. Similar results were obtained with 0.08 m̈g/ml actinomycin D. Protein synthesis and mitotic activity were also affected by lucanthone but the drug did not markedly interfere with DNA synthesis.

Lucanthone appeared to be much less effective in cell killing than actinomycin D and its inhibitory effects on the nucleolar RNA synthesis and other cellular processes proved readily reversible. The results allow to conclude that lucanthone may be useful as a tool for studying RNA synthesis in animal cells.

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