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The mutational origin of serum independence in Chinese hamster cells in vitro

✍ Scribed by Nina B. Varshaver; Marina I. Marshak; N. I. Shapiro

Publisher
John Wiley and Sons
Year
1983
Tongue
French
Weight
467 KB
Volume
31
Category
Article
ISSN
0020-7136

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✦ Synopsis

Abstract

The genetic mechanisms determining the ability of transformed cells to grow in a medium with a low serum content (ser^+^) were studied in a clone of Chinese hamster cells with normal serum requirements. The fluctuation test has shwon that serum independence occurs as a random spontaneous event. Its rate of occurrence is about 10^βˆ’5^. The concomitant study of a gene mutation (resistance to 6‐mercaptopurine ‐ 6MP) revealed similar characteristics with respect to the distribution of the number of mutants in replicate cultures. N‐methyl‐N^1^‐nitro‐N‐nitrosoguanidine (MNNG) and SV40 significantly increased the frequency of ser^+^ colonies. Induction was detected after an expression time of 3‐4 days, which is typical of gene mutations. In 16 out of 18 ser^+^ clones of independent origin the ser^+^ character remained stable. The results suggest that the ser^+^ character originates in most cases from a mutation event.

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