Northern Blots for Small RNAs and MicroRNAs
β Scribed by Rio, D. C.
- Book ID
- 127134391
- Publisher
- Cold Spring Harbor Laboratory Press
- Year
- 2014
- Tongue
- English
- Weight
- 247 KB
- Volume
- 2014
- Category
- Article
- ISSN
- 1940-3402
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π SIMILAR VOLUMES
## Abstract Small noncoding RNAs are key controllers of cellular function, and their deregulation can lead to cancer development and metastatic evolution. This review summarizes the most important examples of small RNAs involved in human cancer and discusses their clinical use as biomarkers and dru
We describe a modification of the Northern technique that allows the detection of RNA either native and/or containing hidden breaks. We found that the highest sensitivity of the hybridization signals was obtained after denaturation of the RNA in the gel prior to its transfer onto a nylon membrane (G