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A procedure for Northern blot analysis of native RNA

✍ Scribed by Edouard W. Khandjian; Claude Méric

Publisher
Elsevier Science
Year
1986
Tongue
English
Weight
835 KB
Volume
159
Category
Article
ISSN
0003-2697

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✦ Synopsis

We describe a modification of the Northern technique that allows the detection of RNA either native and/or containing hidden breaks. We found that the highest sensitivity of the hybridization signals was obtained after denaturation of the RNA in the gel prior to its transfer onto a nylon membrane (GeneScreen) followed by uv irradiation. The sensitivity of the method using native RNA was found to be equivalent to that obtained with denatured RNA. o 1986 Academic ~ss, 1~.

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