𝔖 Bobbio Scriptorium
✦   LIBER   ✦

A simple procedure for fractionation of transfer RNA

✍ Scribed by Endre Egyházi

Publisher
Elsevier Science
Year
1970
Tongue
English
Weight
298 KB
Volume
33
Category
Article
ISSN
0003-2697

No coin nor oath required. For personal study only.

✦ Synopsis

Interest in the biology of transfer RNA (tRNA) has led to the development of a large number of methods for preparative isolation and analytical characterization of different tRNA species such as countercurrent distribution between two phases (1)) partition chromatography (2)) chromatography on columns of hydroxylapatite ( 3)) methylated albumin-kieselguhr ( 4)) DEAE-cellulose ( 5)) and benzoylated DEAEcellulose ( 6)) as well as chemical procedures involving oxidation of amino acid free tRNA with periodate and separation of the formed dialdehyde tRNA from aminoacyl tRNA (7). In the present communication a simple chromatographic procedure will be described based on the use of methylated albumin coated on nitrocellulose membrane filters (MANM).

This technique is capable of resolving individual unacylated tRNA species partially or completely from a crude mixture of Esche-rich&z coli 4 S RNA species. The procedure eliminates a number of laborious steps involved in the conventional column chromatography such as packing and washing of column and is time-saving due to small volumes of eluting gradient solutions.

MATERIALS AND METHODS

📜 SIMILAR VOLUMES

A simple procedure for study of ribosoma
A simple procedure for study of ribosomal and transfer RNA of Drosophila melanogaster
✍ John K. Tomkins; T. Billington 📂 Article 📅 1972 🏛 Elsevier Science 🌐 English ⚖ 306 KB

DrosophiZa is increasingly being used as an experimental animal for molecular studies of gene expression in higher organisms. The ribosomal RNA and transfer RNA gene products of Drosophila have proved especially useful for such studies. They are major cellular products synthesized from genes which h

A simple and inexpensive procedure for p
A simple and inexpensive procedure for preparative polyacrylamide gel electrophoresis of RNA
✍ Allan Jacobson; Harvey F. Lodish 📂 Article 📅 1973 🏛 Elsevier Science 🌐 English ⚖ 249 KB

## A method that is both simple and inexpensive is described for preparative polyacrylamide gel electrophoresis of low-molecular-weight RNA. The utility of the method is demonstrated by the preparative separation of 4S and 5s RX-i.

A simple screening procedure for microbi
A simple screening procedure for microbial phase-transfer mediators conveying anions
✍ C. Stengel; G. Reinhardt; Dr. U. Gräfe 📂 Article 📅 1992 🏛 John Wiley and Sons 🌐 English ⚖ 446 KB

## Abstract The conveyance of methylorange (helianthin) anion from a weakly acidic aqueous solution to a toluene layer was taken as a measure for the presence of anionophoric metabolites in microbial culture extracts mediating the transfer of amphiphilic anions from the water phase to an organic so

A simple design for a fraction collector
A simple design for a fraction collector
✍ John W. Mehl 📂 Article 📅 1963 🏛 Elsevier Science 🌐 English ⚖ 240 KB

Although there are some fraction collectors available for large volumes and for operation under refrigeration, the instrument described here has the advantage of being simple and readily fabricated without any demanding machine work. It is also sufhciently compact that the space required in a latera

A simplified procedure for fractionating
A simplified procedure for fractionating plant materials
✍ William M. Laird; Eddie I. Mbadiwe; Richard L. M. Synge 📂 Article 📅 1976 🏛 John Wiley and Sons 🌐 English ⚖ 267 KB

## Abstract Sequential extraction of plant materials with methanol–chloroform–water and phenol–acetic acid–water mixtures gave good yields of water‐soluble low‐molecular‐mass substances, lipids, proteins and polysaccharides in separate fractions unlaboriously and with little chemical damage. Result